Transcriptomic analysis identified other RNAs that are regulated by a similar mechanism. HuR and PARN destabilize Ctn RNA in absence of ADAR2, indicating that ADAR2 stabilizes Ctn RNA by antagonizing its degradation by PARN and HuR. Unedited Ctn RNA shows enhanced interaction with the RNA-binding proteins HuR and PARN. Furthermore, ADAR2-mediated stabilization of Ctn RNA occurred in an editing-independent manner. In absence of ADAR2, the abundance and half-life of Ctn RNA are significantly reduced. ADAR2 interacts with and edits the 3Î.,UTR of nuclear-retained Cat2 transcribed nuclear RNA (Ctn RNA).
Here, we demonstrate that association of ADAR2 with RNA stabilizes a subset of transcripts. The significance of editing within non-coding regions of RNA is poorly understood. In summary, we identify a regulatory mechanism whereby ADAR2 enhances target RNA stability by limiting the interaction of RNA-destabilizing proteins with their cognate substrates.ĪB - Adenosine deaminases acting on RNA (ADARs) catalyze the editing of adenosine residues to inosine (A-to-I) within RNA sequences, mostly in the introns and UTRs (un-translated regions).
N2 - Adenosine deaminases acting on RNA (ADARs) catalyze the editing of adenosine residues to inosine (A-to-I) within RNA sequences, mostly in the introns and UTRs (un-translated regions). T1 - ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins In summary, we identify a regulatory mechanism whereby ADAR2 enhances target RNA stability by limiting the interaction of RNA-destabilizing proteins with their cognate substrates.", ADAR2 interacts with and edits the 3.,UTR of nuclear-retained Cat2 transcribed nuclear RNA (Ctn RNA).
In summary, we identify a regulatory mechanism whereby ADAR2 enhances target RNA stability by limiting the interaction of RNA-destabilizing proteins with their cognate substrates.Ībstract = "Adenosine deaminases acting on RNA (ADARs) catalyze the editing of adenosine residues to inosine (A-to-I) within RNA sequences, mostly in the introns and UTRs (un-translated regions).
Adenosine deaminases acting on RNA (ADARs) catalyze the editing of adenosine residues to inosine (A-to-I) within RNA sequences, mostly in the introns and UTRs (un-translated regions).
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